![]() Bottom panels Not1-treated chromosomes from: D – non inverted strains, recB (JJC5826) and recA recD (JJC5912 cured of pAM-RecA+) E – InvA (JJC4010). C - InvBE recJ (JJC5852), InvBE recD (JJC5898) and InvBE recA (JJC5911 cured of pAM-RecA+). Top panels I-Sce1-treated chromosomes from: A - Non-inverted strains, Rec+ (wt, JJC5823), recB (JJC5826) and recA recD (JJC5912 cured of pAM-RecA+) B - InvA (JJC5891). Representative examples of these control lanes are shown here. Each PFGE gel, and consequently each membrane used for Southern blotting, carried at least one such control, that indeed showed no fork breakage, as shown here. For all experiments shown in Figure 3, Figure 4, Figure 5, Figure 6, plugs of a control strain expected to show no fork breakage (a non-inverted recB or recA recD mutant, a InvA or InvBE RecBC + mutant) were prepared in parallel with plugs of Inv recBC or Inv recA recD mutants. Figure S1: Control strains do not produce linear DNA when shifted to rich medium.
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